Effect of Rapamycin on Expression of Survivin and Caspase-3 and Its Influence on K562 Cell Ultrastructure / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 52-55, 2016.
Article
in Zh
| WPRIM
| ID: wpr-272506
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of rapamycin on the expression of survivin and caspase-3 at mRNA level in K562 cells and the influence of rapamycin on K562 cell ultrastructure.</p><p><b>METHODS</b>The effects of rapamycin at various concentration on K562 cell proliferation were analyzed by CCK8; the morphological characteristics of K562 cells was observed by transmission electron microscopy; the expression of survivin and caspase-3 at mRNA level in K562 cells treated with rapamycin was detected by RT-PCR.</p><p><b>RESULTS</b>The proliferation of K562 cells was significantly inhibited by rapamycin. The apoptosis level of K562 cells increased with increase of rapamycin concentration, the expression of survivin at mRNA level decreased with increase of rapamycin concentration (P < 0.05). The expression of caspase-3 at mRNA level increased with increase of rapamycin concentration.</p><p><b>CONCLUSION</b>Rapamycin can prornote K562 cell apoptosis through up-regulating caspase-3 level and reduceing survivin level.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
/
Apoptosis
/
K562 Cells
/
Sirolimus
/
Cell Proliferation
/
Inhibitor of Apoptosis Proteins
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Caspase 3
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article