Your browser doesn't support javascript.
loading
Magnetic Nanoparticles of Fe3O4 Enhance Artesunate-inducing Apoptosis of SKM-1 Cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1647-1651, 2015.
Article in English | WPRIM | ID: wpr-272545
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of the copolymer of magnetic nanoparticles of Fe(3)O(4) (MNPs-Fe(3)O(4)) and artesunate (ART) on myelodysplastic syndromes (MDS) cell line SKM-1 cells and the potential mechanisms.</p><p><b>METHODS</b>The protein expression levels of BCL-2, BAX, Caspase-3, and Survivin in SKM-1 cells treated with or without the co-polymer were measured by Western blot. The co-polymer-induced apoptosis rate of SKM-1 cells was measured by flow cytometry.</p><p><b>RESULTS</b>The apoptosis rate of SKM-1 cells in the copolymer groups was higher than that in both MNPs-Fe(3)O(4) and artesunate groups alone. The MNPs-Fe(3)O(4) may enhance ART-induced cell apoptosis. Western blot assay showed that the expression of survivin and BCL-2 protein were down-regulated in the ART group, and this down-regulation was even more significant in the group of copolymer of ART with MNPs-Fe(3)O(4). The levels of BAX were increased both in ART group and the copolymer of ART with MNPs-Fe(3)O(4) group, as compared with control group and MNPs-Fe(3)O(4) group. The levels of active-caspase-3 were obviously up-regulated when the ART was combined with the MNPs-Fe(3)O(4). The copolymer of ART with MNPs-Fe(3)O(4) could trigger changes in the expression levels of apoptosis-related genes in SKM-1 cells, among which up-regulation of BAX and down-regulation of survivin and BCL-2 are the 2 major alterations.</p><p><b>CONCLUSION</b>Artesunate can induce the apoptosis of SKM-1 cells, and MNPs-Fe(3)O(4) may enhance the cell apoptosis induced by ART.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Ferric Compounds / Down-Regulation / Up-Regulation / Apoptosis / Artemisinins / Cell Line, Tumor / Inhibitor of Apoptosis Proteins / Caspase 3 / Magnetite Nanoparticles Limits: Humans Language: English Journal: Journal of Experimental Hematology Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Ferric Compounds / Down-Regulation / Up-Regulation / Apoptosis / Artemisinins / Cell Line, Tumor / Inhibitor of Apoptosis Proteins / Caspase 3 / Magnetite Nanoparticles Limits: Humans Language: English Journal: Journal of Experimental Hematology Year: 2015 Type: Article