Emodin Induces Apoptosis of K562/Adr Cells Probably through Akt-Caspase 3 Signal Pathway / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1556-1559, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-272562
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effects of emodin on multidrug resistant leukemia cell line K562/Adr, and to explore the role of Akt-Caspase 3 signal pathway in apoptosis of K562/Adr cells treated with emodin.</p><p><b>METHODS</b>K562/Adr cells were exposed to emodin of different doses. The ability of emodin to induce apoptosis of K562/Adr cells was detected by Annexin V/PI double labeled flow cytometry and DNA ploidy analysis, the expressions of procaspase-3, PARP, Akt, p-Akt protein were determined by Western blot.</p><p><b>RESULTS</b>Apoptosis in K562/Adr cells could be induced by emodin in a dose dependent manner, Western blot results showed that emodin down-regulated the expression levels of procaspase-3, Akt, p-Akt, PARA 116 KD in treated K562/Adr cells, up-regulated expressions leves of PARP 85 KD in a time-dependent manner.</p><p><b>CONCLUSION</b>The Akt-Caspase 3 signal pathway may be involved in these processes.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Signal Transduction
/
Down-Regulation
/
Emodin
/
Apoptosis
/
K562 Cells
/
Caspase 3
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS