Determination of active anthraquinones in Rheum and its tea preparations by micellar electrokinetic capillary electrophoresis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 870-873, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-272779
ABSTRACT
<p><b>OBJECTIVE</b>To establish an instant determination method of emodin, aloe-emodin and rhein, from Rheum, and one of their preparations, Qinghai Wild Dahuang Tea, by micellar electrokinetic capillary electrophoresis for the first time.</p><p><b>METHOD</b>Separation was carried out in an uncoated fused silica capillary (75 microm x 50.0 cm). Meanwhile, a running voltage 20 kV, 15.0 mmol x L(-1) borax buffer with 30.0 mmol x L(-1) SDS and 10% ethanol (pH 9.60) and a UV detector at 254 nm were adopted.</p><p><b>RESULT</b>The linear calibration rang was 4-120 mg x L(-1) (r = 0.992 1) for emodin, 10-200 mg x L(-1) (r = 0.997 0) for aloe-emodin, and 2-100 mg x L(-1) (r = 0.997 1) for rhein, respectively. Under the optimum conditions, the relative standard deviation (RSD) values (n = 6) for the migration time and the peak area of each peak were 0.59%-0.80%, 1.30%-3.22%, respectively. The contents of the analytes were easily determined with recoveries ranging from 97.6%-102.3%.</p><p><b>CONCLUSION</b>The method is proved to be simple, rapid and accurate, and can be used for the quality control of medicinal herb, Rheum, and its tea preparation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Rheum
/
Chemistry
/
Anthraquinones
/
Emodin
/
Chromatography, Micellar Electrokinetic Capillary
/
Plant Preparations
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2004
Type:
Article
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