Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.</p><p><b>METHOD</b>Calli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.</p><p><b>RESULT AND CONCLUSION</b>After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.</p>