Effect of leptin on bone metabolism in rat model of traumatic brain injury and femoral fracture / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 7-13, 2011.
Article
in English
| WPRIM
| ID: wpr-272881
ABSTRACT
<p><b>OBJECTIVE</b>To observe serum and callus leptin expression within the setting of fracture and traumatic brain injury (TBI).</p><p><b>METHODS</b>A total of 64 male SD rats were randomized equally into 4 groups nonoperated group, TBI group, fracture group, and fracture+TBI group. Rats were sacrificed at 2, 4, 8 and 12 weeks after fracture+TBI. Serum leptin was detected using radioimmunoassay, and callus formation was measured radiologically. Callus leptin was analyzed by immunohistochemistry.</p><p><b>RESULTS</b>Serum leptin levels in the fracture group, TBI group and combined fracture+TBI group were all significantly increased compared with control group at the 2 week time-point (P less than 0.05). Serum leptin in the combined fracture +TBI group was significantly higher than that in the fracture and TBI groups at 4 and 8 weeks after injury (P less than 0.05). The percentage of leptin-positive cells in the fracture+TBI callus and callus volume were significantly higher than those in the fracture-only group (P less than 0.01).</p><p><b>CONCLUSIONS</b>We demonstrated elevated leptin expression within healing bone especially in the first 8 weeks in a rat model of fracture and TBI. A close association exists between leptin levels and the degree of callus formation in fractures.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Pathology
/
Physiology
/
Blood
/
Brain Injuries
/
Immunohistochemistry
/
Rats, Sprague-Dawley
/
Fracture Healing
/
Leptin
/
Femoral Fractures
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2011
Type:
Article
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