Dexamethasone inhibits osteoblastic differentiation by down-regulation of LIM mineralization protein 1 / 生理学报
Acta Physiologica Sinica
;
(6): 33-37, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-272988
ABSTRACT
To investigate the mechanisms of the inhibition of osteoblastic differentiation by dexamethasone (DEX), the effects of different doses of DEX on the activity of alkaline phosphatase (ALP), the synthesis of osteocalcin (OC) and the expression of collagen type I were observed in the cultured rat osteoblasts. The LIM mineralization protein-1 (LMP-1) mRNA, a positive regulator of osteoblasts, was semi-quantified by RT-PCR. The results showed that a lower dose (10(-9) mol/L) of DEX could enhance the activity of ALP, the synthesis of OC and the expression of collagen type I. However, a higher dose (10(-7) mol/L) of DEX inhibited them and down-regulated the expression of LMP-1 mRNA in osteoblasts. It is suggested that DEX stimulates osteoblast differentiation at lower dose, while at higher dose it inhibits osteoblast differentiation. The inhibitory action of DEX on osteoblast differentiation might be mediated by the down-regulation of LMP-1 mRNA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
Transcription Factors
/
RNA, Messenger
/
Dexamethasone
/
Down-Regulation
/
Cell Differentiation
/
Cells, Cultured
/
Homeodomain Proteins
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2002
Type:
Article
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