Inhibitory Effect of Antibody to alphavbeta5 in Corneal Angiogenesis
Journal of the Korean Ophthalmological Society
;
: 1594-1599, 2001.
Article
in Korean
| WPRIM
| ID: wpr-27310
ABSTRACT
PURPOSE:
This study investigated the importance of alphavbeta5 function during vascular endothelial growth factor (VEGF) induced corneal angiogenesis by examining the effects of antibody to alphavbeta5 that blocks alphav 5-mediated cell adhesion to vitronectin.METHODS:
A hydrogel disk containing 500 ng of VEGF was implanted into the superior corneal stroma of each of sixteen New Zealand white rabbit eyes. Each eye also received a second hydrogel disk placed adjacent to the first, randomized to contain either 40 g of antibody to alphavbeta5 (n=8) or phosphate-buffered saline (PBS)(n=8). Both disks were positioned 1.2 mm apart from the superior limbus. Eyes were examined daily under a stereomicroscope by two observers and assigned an angiogenesis score based on number and length of new blood vessels.RESULTS:
On days 3 through 7 postimplantation, angiogenesis scores were significantly lower in eyes treated with antibody to alphavbeta5 (averaged score=16.33) as compared to eyes treated with PBS (averaged score=26.52)(P<0.05, Wilcoxon signed rank test).CONCLUSIONS:
In a rabbit corneal micropocket assay, antibody to alphavbeta5 inhibits corneal angiogenesis induced by VEGF. Substances that target the integrin alphavbeta5 subunit may have therapeutic potential in disorders characterized by ocular neovascularization.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Vessels
/
Cell Adhesion
/
Corneal Neovascularization
/
Vitronectin
/
Corneal Stroma
/
Hydrogels
/
Vascular Endothelial Growth Factor A
/
New Zealand
Type of study:
Controlled clinical trial
Country/Region as subject:
Oceania
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS