Tissue engineering of dentin-pulp complex-like structures by human dental mesenchymal cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 408-411, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-273201
ABSTRACT
<p><b>OBJECTIVE</b>To establish three-dimensional culture model of human dental mesenchymal cells and bioengineer in vivo with ceramic bovine bone (CBB) and Collagraft as scaffolds.</p><p><b>METHODS</b>Human dental mesenchymal cells induced upon stimulation of bFGF and IGF-1 or TGF-beta(1) were implanted onto CBB and Collagraft containing the same kinds of growth factors respectively. Then cell/scaffold constructs were transplanted into nude mice to establish in vivo culture model of dental mesenchymal cells. Control groups were set up at the same time. After 4 weeks or 10 weeks, the implants were taken out for histological and immunohistochemical analysis.</p><p><b>RESULTS</b>Within 10-week implant tissues, typical dentin-pulp complex-like structures were generated in scaffolds containing growth factors. Human dentin sialoprotein (DSP) was expressed in the newly formed dentin. This phenomenon wasn't observed in control groups and 4-week implants.</p><p><b>CONCLUSIONS</b>Dentin-pulp complex-like structures could be bioengineered successfully with human dental mesenchymal cells and CBB or Collagrafts containing growth factors in nude mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tooth, Deciduous
/
Calcium Phosphates
/
Cells, Cultured
/
Embryology
/
Collagen
/
Cell Biology
/
Tissue Engineering
/
Dental Pulp
/
Dentin
/
Mesenchymal Stem Cells
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2005
Type:
Article
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