In vitro study on cranial neural crest differentiating into ectomesenchymal cell of the first branchial arch by FGF-8 / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 319-322, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-273227
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of FGF-8 on cranial neural crest cell (CNCC) differentiating into ectomesenchymal cell of the first branchial arch, and determine the appropriate dose and stage of CNCC exposure to FGF-8.</p><p><b>METHODS</b>Cranial neural crest explants were cultured in free-serum medium containing modified DMEM/F12 and different doses of FGF-8. The differentiation type of CNCC were determined by in situ hybridization for Hoxa2 and immunocytochemistry for vimentin.</p><p><b>RESULTS</b>Pre-emigrating CNCC demonstrated the negative Hoxa2 stain and positive vimentin stain after treated by 100 ug/FGF-8. Both post-emigrating CNCC group and control group were positive for Hoxa2 and vimentin stain.</p><p><b>CONCLUSIONS</b>On the early stage of CNCC emigration, the first branchial arch phenotype of CNCC could be induced by FGF-8. This experiment could provide in vitro model for study on the mechanism of tooth-jaw regeneration.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Branchial Region
/
Cell Differentiation
/
Cells, Cultured
/
Cranial Nerves
/
Cell Biology
/
Fibroblast Growth Factor 8
/
Mesoderm
/
Mice, Inbred Strains
/
Neural Crest
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2005
Type:
Article
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