Preliminary study on anti-periodontitis immunization with DNA vaccine / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 310-313, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-273229
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protection against periodontal bone loss in the Sprague-Dawley (SD) rats periodontitis model, with the recombined plasmid pcDNA3.1+/kgpcd as DNA gene vaccine.</p><p><b>METHODS</b>PcDNA3.1+/kgpcd was delivered into rats by submandibular gland-targeted injection. The anti-KGPcd sIgA in saliva was measured by indirect ELISA method. Immunohistochemistry staining was used to assess the protection in the animal model.</p><p><b>RESULTS</b>The level of specific anti-KGPcd sIgA in saliva of the experimental group was significantly higher than that of control group. HE staining showed that immunization with recombined plasmid pcDNA3.1+/kgpcd could protect or minimize tissue destruction caused by subsequent P. gingivalis challenge in the rat model.</p><p><b>CONCLUSIONS</b>The results indicate that pcDNA3.1+/kgpcd was a good candidate for anti-periodontitis gene vaccine and could provide protection against Porphyromonas gingivalis-caused periodontitis in rat lesion model.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Periodontitis
/
Immunoglobulin A, Secretory
/
Bacterial Vaccines
/
Rats, Sprague-Dawley
/
Porphyromonas gingivalis
/
Vaccines, DNA
/
Therapeutic Uses
/
Allergy and Immunology
/
Genetics
/
Microbiology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2005
Type:
Article
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