Effects of 17β-estradiol on proliferation and migration of MCF-7 cell by regulating expression of claudin-6 / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 44-47, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-273424
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of estrogen in the regulation of the expression of claudin-6 and biological behavior in MCF-7 cells.</p><p><b>METHODS</b>RT-PCR and immunocytochemistry were conducted to analyze the expression and localization of claudin-6 in MCF-7 cells treated with 17β-estradiol. CCK-8 kit assay and Scratch Test were conducted to analyze the capability of proliferation and migration of 17β-estradiol treated MCF-7 cells.</p><p><b>RESULTS</b>RT-PCR analysis and immunocytochemistry showed that 17β-estradiol induced a concentration-and time-dependent effect on claudin-6. At 5 nmol/L and at 24 h, 17β-estradiol treatment led to an increased level of claudin-6, which was located in the membrane of MCF-7 cells. CCK-8 analysis showed a significant decrease in the capability of proliferation of MCF-7 cells compared with the control group (P < 0.05). Cells Scratch Test showed decreased migration capability of MCF-7 cells compared with the control group (P > 0.05).</p><p><b>CONCLUSIONS</b>17β-E2 might regulate the expression of claudin-6 and inhibit the proliferation and migration of MCF-7 cells. The inhibitory effects of 17β-E2 on growth and migration of MCF-7 cells may be mediated by claudin-6 expression regulation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Breast Neoplasms
/
Cell Movement
/
Cell Line, Tumor
/
Cell Proliferation
/
Dose-Response Relationship, Drug
/
Estradiol
/
Claudins
/
Membrane Proteins
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2010
Type:
Article
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