Construction of rat bowel transforming growth factor beta1 recombinant adenovirus vector / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 76-79, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-273883
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant adenovirus vector which regulates the expression of rat transforming growth factor beta (TGF-beta1).</p><p><b>METHODS</b>Total RNA was extracted from F344/N rat small intestine pre-treated with Con A to clone TGF-beta1. pTRE-shuttle vector was used as mediator to ligate TGF-beta1 gene and backbone of replication-incompetent adenoviral vector. The constructed recombinant adenovirus contained tetracycline-responsive element which could regulate the expression of inserted genes. After identification, the desired recombinant adenovirus was packaged in HEK 293 cells. Supernatant of high titer adenovirus was collected to detect the TGF-beta1 gene expression by green fluorescent protein(GFP).</p><p><b>RESULTS</b>The constructed recombinant adenovirus was identified by restriction endonucleases cutting, sequencing, PCR and GFP examination.</p><p><b>CONCLUSION</b>Rat TGF-beta1 recombinant adenovirus is established successfully, which provides material and evidence for further research of dendritic cell (DC) modified by TGF-beta1 to induce immune tolerance in rat heterotopic small bowel transplantation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombination, Genetic
/
Dendritic Cells
/
Transfection
/
Gene Expression
/
Cells, Cultured
/
Adenoviridae
/
Cloning, Molecular
/
Transforming Growth Factor beta1
/
Genetic Vectors
/
Genetics
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2008
Type:
Article
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