Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 4022-4028, 2011.
Article
in English
| WPRIM
| ID: wpr-273932
ABSTRACT
<p><b>BACKGROUND</b>The seed cell is a core problem in bone tissue engineering research. Recent research indicates that human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts in vitro, which suggests that they may become a new kind of seed cells for bone tissue engineering. The aim of this study was to evaluate the osteogenic differentiation of hDPSCs in vitro and bone-like tissue formation when transplanted with three-dimensional gelatin scaffolds in vivo, and hDPSCs may become appropriate seed cells for bone tissue engineering.</p><p><b>METHODS</b>We have utilized enzymatic digestion to obtain hDPSCs from dental pulp tissue extracted during orthodontic treatment. After culturing and expansion to three passages, the cells were seeded in 6-well plates or on three-dimensional gelatin scaffolds and cultured in osteogenic medium. After 14 days in culture, the three-dimensional gelatin scaffolds were implanted subcutaneously in nude mice for 4 weeks. In 6-well plate culture, osteogenesis was assessed by alkaline phosphatase staining, Von Kossa staining, and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the osteogenesis-specific genes type I collagen (COL I), bone sialoprotein (BSP), osteocalcin (OCN), RUNX2, and osterix (OSX). In three-dimensional gelatin scaffold culture, X-rays, hematoxylin/eosin staining, and immunohistochemical staining were used to examine bone formation.</p><p><b>RESULTS</b>In vitro studies revealed that hDPSCs do possess osteogenic differentiation potential. In vivo studies revealed that hDPSCs seeded on gelatin scaffolds can form bone structures in heterotopic sites of nude mice.</p><p><b>CONCLUSIONS</b>These findings suggested that hDPSCs may be valuable as seed cells for bone tissue engineering. As a special stem cell source, hDPSCs may blaze a new path for bone tissue engineering.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteogenesis
/
Physiology
/
Stem Cells
/
Bone and Bones
/
Cells, Cultured
/
Cell Biology
/
Tissue Engineering
/
Dental Pulp
/
Tissue Scaffolds
/
Methods
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2011
Type:
Article
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