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Human dental pulp stem cell is a promising autologous seed cell for bone tissue engineering / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 4022-4028, 2011.
Article in English | WPRIM | ID: wpr-273932
ABSTRACT
<p><b>BACKGROUND</b>The seed cell is a core problem in bone tissue engineering research. Recent research indicates that human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts in vitro, which suggests that they may become a new kind of seed cells for bone tissue engineering. The aim of this study was to evaluate the osteogenic differentiation of hDPSCs in vitro and bone-like tissue formation when transplanted with three-dimensional gelatin scaffolds in vivo, and hDPSCs may become appropriate seed cells for bone tissue engineering.</p><p><b>METHODS</b>We have utilized enzymatic digestion to obtain hDPSCs from dental pulp tissue extracted during orthodontic treatment. After culturing and expansion to three passages, the cells were seeded in 6-well plates or on three-dimensional gelatin scaffolds and cultured in osteogenic medium. After 14 days in culture, the three-dimensional gelatin scaffolds were implanted subcutaneously in nude mice for 4 weeks. In 6-well plate culture, osteogenesis was assessed by alkaline phosphatase staining, Von Kossa staining, and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the osteogenesis-specific genes type I collagen (COL I), bone sialoprotein (BSP), osteocalcin (OCN), RUNX2, and osterix (OSX). In three-dimensional gelatin scaffold culture, X-rays, hematoxylin/eosin staining, and immunohistochemical staining were used to examine bone formation.</p><p><b>RESULTS</b>In vitro studies revealed that hDPSCs do possess osteogenic differentiation potential. In vivo studies revealed that hDPSCs seeded on gelatin scaffolds can form bone structures in heterotopic sites of nude mice.</p><p><b>CONCLUSIONS</b>These findings suggested that hDPSCs may be valuable as seed cells for bone tissue engineering. As a special stem cell source, hDPSCs may blaze a new path for bone tissue engineering.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Physiology / Stem Cells / Bone and Bones / Cells, Cultured / Cell Biology / Tissue Engineering / Dental Pulp / Tissue Scaffolds / Methods Limits: Animals / Humans Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Osteogenesis / Physiology / Stem Cells / Bone and Bones / Cells, Cultured / Cell Biology / Tissue Engineering / Dental Pulp / Tissue Scaffolds / Methods Limits: Animals / Humans Language: English Journal: Chinese Medical Journal Year: 2011 Type: Article