Study on extraction and immune regulation of polysaccharides from Marsdenia tenacissima / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 457-460, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-274325
ABSTRACT
<p><b>OBJECTIVE</b>To optimize the extraction technology and determine the immune activity of polysaccharides from Marsdenia tenacissima.</p><p><b>METHOD</b>The optimum extraction technology of polysaccharide from M. tenacissima was detected by orthogonal experiments with the extraction rate of polysacchride and the total sugar content as indicators. The immunocompromised mice model was established by intraperitoneal injection cyclophosphamide to detected the content of IL-2, IL-6 in serum, CD4+, CD8+ in the peripheral blood by ELISA and flow cytometry, respectively.</p><p><b>RESULT</b>By the extraction rate of polysaccharide, the sequence of seriousness of all affecting factors from high to low was extracting times, temperature, heating time and water ratio. By the total sugar content, the sequence was temperature, extracting times, water ratio and heating time. Compared with the model group, the pleen index, IL-2, IL-6, CD4/CD8+ were increased significantly (P < 0.05) in the 0.14 g x kg(-1) group and 0. 28 g x kg(-1) group.</p><p><b>CONCLUSION</b>T he optimum extraction condition was as follows extraction three times/1.5 hours at 100 degrees C with 18 ratio of M. tenacissima to water. The polysaccharide of M. tenacissima can enhance the cellular immune and humoral immune.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polysaccharides
/
Spleen
/
Temperature
/
Time Factors
/
Blood
/
Body Weight
/
Chemistry
/
Interleukin-6
/
Interleukin-2
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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