Phamacokinetic study of bergapten in rats plasma by LC-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 381-383, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-274338
ABSTRACT
<p><b>OBJECTIVE</b>To determine bergapten's concentration in plasma and observe its pharmacokinetics in rats using a combined LC-MS/MS analytical method.</p><p><b>METHOD</b>Blood samples were separated on a Hypersil ODS column (4.6 mm x 250 mm, 5 mm) at a temperature of 30 degrees C, and mobile phase consisted of water and methanol (22.5 77.5) at a flow rate of 0.8 mL x min(-1).</p><p><b>RESULT</b>The methodological study showed a good linear relationship of 8.12-162.4 g x L(-1) (r = 0.9999). The inner and inter-days relative standard deviations were both less than 10% , indicating legitimate precise and accuracy to the requirement of biological sample analysis.</p><p><b>CONCLUSION</b>The method is suitable for in vivo quantitative analysis for bergapten due to its accuracy, sensitivity and specificity. The pharmacokinetic process in rats forms a two-compartment model with first-order absorption.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reference Values
/
Time Factors
/
Blood
/
Pharmacokinetics
/
Reproducibility of Results
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Rats, Sprague-Dawley
/
Tandem Mass Spectrometry
/
Methoxsalen
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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