AFLP analysis of genetic diversity of Evodia rutaecarpa / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 278-282, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-274360
ABSTRACT
<p><b>OBJECTIVE</b>To study the genetic background of Evodia rutaecarpa by AFLP, and analyze the genetic diversity of E. rutaecarpa from different areas.</p><p><b>METHOD</b>E. rutaecarpa genomic DNA was extracted. The AFLP reaction system was established and AFLP primer pairs were chosen for the analysis. Forty-six individuals of E. rutaecarpa which from five provinces were analyzed by AFLP. The NTSYS-pc 2.1 software was used for cluster analysis.</p><p><b>RESULT</b>Six out of the original 72 pairs of primers were optimized for the study; AFLP analysis revealed the similarity coefficient of 0.53, the samples of E. rutaecarpa var. officinalis from Zhejiang province was separated from other accessions; E. rutaecarpa var. officinalis also showed more pronounced genetic variation than the E. rutaecarpa, and strong geo-related relevance.</p><p><b>CONCLUSION</b>Variance of genetic background of E. rutaecarpa are large, AFLP analysis method can obviously identify different varieties of E. rutaecarpa, and can detect the genetic characteristics of inter-regional differences.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Genetic Variation
/
Classification
/
DNA, Plant
/
Evodia
/
Amplified Fragment Length Polymorphism Analysis
/
Genetics
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Type:
Article
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