Effect of down-regulation of GAT-1 on analgesic action of oxysophoridine / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3315-3318, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-274377
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of GABA transporter (GAT-1) on the analgesic action of oxysophoridine (OSR) in the central nervous system of mice.</p><p><b>METHOD</b>Hot plate test was used to observe and analyze the effect of gamma aminobutyric acid and the inhibitor of GAT-1 (NO-711) on the analgesic action of oxysophoridine. Real time RT-PCR was used to investigate the influence of OSR on the expression of GAT-1 mRNA induced by formalin in spinal cord and brain of mice.</p><p><b>RESULT</b>Both GABA (2.0 mg x kg(-1), icv) and NO-711(0.125 mg x kg(-1), icv) enhanced the analgesic action of OSR (32.0 mg x kg(-1), iv) in the hot plate test, and the latencies was markedly increased (P < 0.05, P < 0.01). OSR (500.0 mg x kg(-1), iv) significantly inhibited the expression of GAT-1 mRNA induced by formalin (P < 0.05).</p><p><b>CONCLUSION</b>GAT-1 was involved in the analgesia effect of OSR and the down-regulation of GAT-1 mRNA enhanced the analgesic effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Spinal Cord
/
Brain
/
RNA, Messenger
/
Down-Regulation
/
Gene Expression Regulation
/
Alkaloids
/
GABA Plasma Membrane Transport Proteins
/
Genetics
/
Analgesics
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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