Effect of cytoskeleton integrity on the expression of c-fos in osteoblasts induced by fluid shear stress / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 681-685, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-274515
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cytoskeleton integrity on the expression of c-fos gene in osteoblasts induced by fluid shear stress.</p><p><b>METHODS</b>BALB/c mouse primary osteoblasts were divided into four groups (according to fluid shear stress loaded or not and cytochalasin D used or not). The Tagman probe real-time PCR and immunofluorescence were performed to detect the expression levels of c-fos mRNA, c-fos protein and cytoskeleton, respectively. The data were analysed using two-way ANOVA.</p><p><b>RESULTS</b>In control group and cytochalasin D group, fluid shear stress could significantly increase the expression levels of c-fos mRNA (0.1637 +/- 0.0303 and 0.0104 +/- 0.0070, respectively) and protein (177.14 +/- 9.37 and 150.95 +/- 6.17, respectively) in osteoblasts, compared with the unloaded osteoblasts of the control group and the cytochalasin D group (0.0057 +/- 0.0021 and 0.0032 +/- 0.0014, respectively for c-fos mRNA, and 117.96 +/- 4.11 and 119.77 +/- 5.19, respectively for protein, P < 0.05). Induced by the fluid shear stress, the expression levels of c-fos mRNA and protein in cytochalasin D group were lower than control group, and the difference had statistical significance (P < 0.05).</p><p><b>CONCLUSIONS</b>The cytoskeleton integrity in osteoblasts was essential to the expression of c-fos gene induced by fluid shear stress.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Pharmacology
/
Physiology
/
Rheology
/
Stress, Mechanical
/
Cytoskeleton
/
RNA, Messenger
/
Cytochalasin D
/
Cells, Cultured
/
Analysis of Variance
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2009
Type:
Article
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