Effect of radiation on the activity and expression of Ca(2+)-Mg(2+)-ATPase in rat masseter muscle / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 479-482, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-274547
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the activity and expression of Ca(2+)-Mg(2+)-ATPase in irradiated rat masseter muscle.</p><p><b>METHODS</b>The rats were irradiated locally with a single dose of 20 Gy X-ray. The activities of Ca(2+)-Mg(2+)-ATPase were measured with colorimetric method. The protein expression of Ca(2+)-Mg(2+)-ATPase was determined by Western blotting and immunohistochemistry.</p><p><b>RESULTS</b>The activities of Ca(2+)-Mg(2+)-ATPase in masseter muscle decreased by approximately 20% and 40% in irradiated rats on days 3 and 30 postirradiation. There was significant difference in the expression of Ca(2+)-Mg(2+)-ATPase protein between irradiated and nonirradiated rats on day 30 postirradiation. Ca(2+)-Mg(2+)-ATPase protein was found in the cytoplasm of masseter muscle.</p><p><b>CONCLUSIONS</b>The decrease of ATPase activity played an important role in the cause of radiation-induced skeletal muscle injury, while there was no significant reduction in the expression of Ca(2+)-Mg(2+)-ATPase protein in irradiated rat masseter muscle.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Radiation Injuries, Experimental
/
Immunohistochemistry
/
Blotting, Western
/
Ca(2/) Mg(2/)-ATPase
/
Cytoplasm
/
Masseter Muscle
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2009
Type:
Article
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