Detection and functional analysis of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with generalized aggressive periodontitis / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 413-415, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-274562
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of proportion and suppression function of CD-4+ CD-25+ regulatory T cells in the peripheral blood of patients with aggressive periodontitis.</p><p><b>METHODS</b>Flow cytometric analysis was used to detect the frequency of CD-4+ CD-25+ regulatory T cells in the peripheral blood of 16 patients with generalized aggressive periodontitis and 17 patients with chronic periodontitis, as well as 17 periodontal healthy controls. Furthermore, CD-4+ CD-25+ regulatory T cells and CD-4+ CD-25- T cells were separated from peripheral blood of each enrolling subject using magnetic cell sorting technology. The direct suppression effect of CD-4+ CD-25+ regulatory T cells on CD-4+ CD-25- T lymphocytes proliferation was performed by the mixed lymphocytes reaction and measured by 3H-thymidine radioactive assay.</p><p><b>RESULTS</b>The patients with generalized aggressive periodontitis had a lower frequency of CD4+ CD-25+ regulatory T cells (9.71 +/- 4.01)% in the peripheral blood than periodontal healthy controls [(14.72 +/- 3.51)%] and chronic periodontitis patients [(17.01 +/- 5.16 )%], P < 0.05. A significant decrease was found in the suppression function of CD-4+ CD-25+ regulatory T cells from peripheral blood of patients with generalized aggressive periodontitis when co-cultured with CD-4+ CD-25- T lymphocytes in the proportion of 2 1, 1 1 and 1 2 as compared with chronic periodontitis patients and periodontal healthy controls (P < 0.05).</p><p><b>CONCLUSIONS</b>Diminished numbers and decreased suppression function of CD-4+ CD-25+ regulatory T cells were found in patients with generalized aggressive periodontitis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aggressive Periodontitis
/
Blood
/
Case-Control Studies
/
T-Lymphocytes, Regulatory
/
Cell Biology
/
Flow Cytometry
Type of study:
Diagnostic study
/
Observational study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2009
Type:
Article
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