Expression of recombinant cytolethal distending toxin of Actinobacillus actinomycetemcomitans / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To examine the expression of recombinant cytolethal distending toxin (CDT) produced by Actinobacillus actinomycetemcomitans (Aa).</p><p><b>METHODS</b>CDT encoding gene cdtABC was amplified by PCR. Through TA clone and restriction endonuclease digestion, gene cdtABC and vector pQE60 were ligated to form pQE60-cdtABC expression system which transformed into competent cells. Protein expression was induced by IPTG and examined by SDS-PAGE and Western-blotting.</p><p><b>RESULTS</b>Random colony PCR of pQE60-cdtABC transformed cells demonstrated that all strains contained cdtABC gene. The DNA sequence was blast with cdtABC gene from GenBank and 99% homology was obtained. SDS-PAGE and Western-blotting confirmed that recombinant CDT was obtained.</p><p><b>CONCLUSIONS</b>CDT protein expression system was reconstructed and recombinant protein was obtained. Actinobacillus actinomycetemcomitans;</p>