Photo-activated DNA binding and antimicrobial activities of alkaloids from Glycosmis pentaphylla / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1646-1652, 2012.
Article
in English
| WPRIM
| ID: wpr-274609
ABSTRACT
In our screening for photosensitizers from natural resources, four alkaloids were isolated from Glycosmis pentaphylla by various chromatography techniques. Their structures were identified as glycoborinine (1), glybomine B (2), carbalexin A (3) and N-p-coumaroyltyramine (4) by spectral analysis. Their photoactivated antimicrobial activities were evaluated by thin-layer chromatography (TLC) agar overlay assay against Staphylococcus aureus and Bacillus subtilis. It was found that compounds 1 and 4 showed photo-activated antimicrobial activities. Meantime, photo-activated DNA binding activities of these compounds were also assessed by using a specially prepared 1.8 kb DNA fragment and restriction enzymes. Under UVA irradiation, compound 1 showed moderate inhibition on Nde I, Xba I, Nco I and Bcl I which have either 5'-TpA or 5'-ApT and trace or no inhibition on other restriction enzymes. It showed a similar inhibition pattern with the reference 8-methoxypsoralen. However, compounds 2-4 showed no inhibition against any of the restriction enzymes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Protein Binding
/
Staphylococcus aureus
/
Bacillus subtilis
/
Ultraviolet Rays
/
Carbazoles
/
Molecular Structure
/
DNA Restriction Enzymes
/
Chemistry
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2012
Type:
Article
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