Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1159-1163, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-274683
ABSTRACT
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pharmacology
/
Plasmids
/
Ribavirin
/
RNA-Dependent RNA Polymerase
/
Transfection
/
Amantadine
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Genes, Reporter
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS