Preparation and identification of monoclonal antibody against alpha-momorcharins / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1181-1184, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-274930
ABSTRACT
Three BALB/c mice were immunized four times with alpha-momorcharins (alpha-MMC). Using polyethylene glycol (PEG) method, the immunized splenocytes were fused with SP2/0 cells. One strain of hybridoma cells was obtained which secrete antibodies against alpha-MMC. To get ascites, the hybridoma cells were injected into the abdominal cavity of mice. The antibodies were purified from ascites. Indirect enzyme linked immunosorbent assay (ELISA) and Western blot assay were applied to determine the specifity of the monoclonal antibody (McAb). The results showed that the McAb was specific to alpha-MMC without detectable cross-activity with MAP30. The McAb provided detecting method for further research of the structure and function of alpha-MMC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Enzyme-Linked Immunosorbent Assay
/
Blotting, Western
/
Allergy and Immunology
/
Ribosome Inactivating Proteins
/
Hybridomas
/
Metabolism
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2011
Type:
Article
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