Antagonistic effect of aqueous extract of detoxified cottonseeds on corticosterone-induced lesion in cultured PC12 cells / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 442-446, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-274965
ABSTRACT
<p><b>OBJECTIVE</b>To study the possible mechanism of aqueous extract of detoxified cottonseeds (CTN-W).</p><p><b>METHOD AND RESULT</b>CTN-W 0.01, 0.03, 0.10, 0.30 mg.mL-1 was incubated directly with the synaptic membrane extracted from the cerebral cortex in rats, and adenylyl cyclase (AC) activity was detected by using radio-immunoassay.</p><p><b>RESULT</b>Showed that CTN-W could activate AC in a dose-dependend manner. After incubation with PC12 cells in the presence of corticosterone 2 x 10(-4)mol.L-1 for 48 h, CTN-W 0.08, 0.4, 2 mg.mL-1 protected PC12 cells from the lesion induced by corticosterone.</p><p><b>CONCLUSION</b>Antidepressant and anxiolytic effects of CTN-W are related with the activation of AC-cAMP pathway in signal transduction system, thus protecting neurons from the lesion. These two aspects maybe partly form the mechanism of CTN-W's action.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Seeds
/
Synaptic Membranes
/
Anti-Anxiety Agents
/
Corticosterone
/
Drugs, Chinese Herbal
/
Chemistry
/
Adenylyl Cyclases
/
PC12 Cells
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2002
Type:
Article
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