Determination of curculigoside in crude medicine Curculigo orchioides by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 192-194, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-274986
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine.</p><p><b>METHOD</b>The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45801) and detect wavelength was set at UV 283 nm.</p><p><b>RESULT</b>The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%.</p><p><b>CONCLUSION</b>This method could be used to control the quality of crude medicine C. orchioides.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Benzoates
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Rhizome
/
Curculigo
/
Glucosides
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2002
Type:
Article
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