Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.</p><p><b>METHOD</b>TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (72.52.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (1585) as mobile phase. The detection wavelength was 327 nm.</p><p><b>RESULT</b>The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.</p><p><b>CONCLUSION</b>The methods and data could be used for quality control.</p>