Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 267-269, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-275015
ABSTRACT
<p><b>OBJECTIVE</b>To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.</p><p><b>METHOD</b>TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (72.52.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (1585) as mobile phase. The detection wavelength was 327 nm.</p><p><b>RESULT</b>The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.</p><p><b>CONCLUSION</b>The methods and data could be used for quality control.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Chemistry
/
Chlorogenic Acid
/
Artemisia
Type of study:
Qualitative research
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2002
Type:
Article
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