Determination of verbascoside in herba of Galeobdolon Chinense by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine Verbascoside in Galeobdolon chinense.</p><p><b>METHOD</b>HPLC method was used, with the column packed with Kromasil C18(4.6 mm x 150 mm, 5 microns). The mobile phase was a mixture of acetonitrile and 1% acetic acid in a ratio of 1387, and the ultraviolet wavelength was set at 350 nm.</p><p><b>RESULT</b>The average recovery was 97.0%, the RSD being 1.69%.</p><p><b>CONCLUSION</b>This method is sensitive and reliable. It can be used to determine being Verbascoside in Galeobdolon chinense.</p>