Expression of biologically active recombinant arresten in Nicotiana tabacum / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1520-1525, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275328
ABSTRACT
In this report, the biological activity of the recombinant Arresten expressed in Nicotiana tabacum was studied. The gene coding for the tumor angiogenesis inhibitor Arresten was PCR-amplified from the plasmid pCA and its plant expression vector named pCAMBIAarr was constructed by inserting the Arresten cDNA fragment into the NcoI/BstEII sites of the plant binary expression vector pCAMBIA1301. Then pCAMBIAarr was transferred into Agrobacterium tumefacien LBA4404 by the freeze-thaw method. The adventitious shoots and regenerated plants of Nicotiana tabacum with hygromycinB-resistance were obtained via Agrobacterium-mediated leaf disk transformation method. Southern hybridization, RT-PCR and Western blotting analysis showed that the Arresten cDNA was integrated into the genome of some of the regenerated plants and the recombinant Arresten was expressed with a molecular size of 26 kD. Recombinant Arresten purified from transgenic tobacco leaves had an anti-proliferative effect on bovine endothelial cells. We speculate that biologically active recombinant Arresten can be produced by using plants as bioreactors.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tobacco
/
Transformation, Genetic
/
Recombinant Proteins
/
Plants, Genetically Modified
/
Agrobacterium tumefaciens
/
Angiogenesis Inhibitors
/
Collagen Type IV
/
Genetic Vectors
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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