Cloning and functional analysis of two regulators involved in regulation of cellulase formation in Trichoderma koningii / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1808-1812, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275336
ABSTRACT
ACEI and Xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in Trichoderma reesei, they compete for a binding site in the xyn1 (Xylanase1-encoding) gene promoter. To further investigate the mechanism for the transcriptional regulation of cellulases, DNA-binding domains of both ACEI and Xyr1 in T. Koningii were expressed from E. coli. They both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assays, suggesting ACEI and Xyr1 not only compete for binding to xyn1 promoter but also to cbh1 promoter.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trichoderma
/
Binding Sites
/
Gene Expression Regulation, Enzymologic
/
Gene Expression Regulation, Fungal
/
Trans-Activators
/
Cellulase
/
Promoter Regions, Genetic
/
Endo-1,4-beta Xylanases
/
Escherichia coli
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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