An enzyme electrode biosensor for inosine determination / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1796-1800, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275338
ABSTRACT
An enzyme electrode biosensor was used for the amperometric determination of inosine in its tablets by co-immobilizing nucleoside phosporylase and xanthine oxidase on a hydrogen peroxide electrode. As a fundamental electrode the hydrogen peroxide electrode has an advantage of stability in analysis compared with the 02 electrode. The enzyme electrode showed a linear response to inosine in the range of 1-268 mg/L with a response of 60 seconds under a sample injection volume of 25 microL. Based on the enzyme electrode, inosine solutions were determined with an average recover rate of 100.8% and a relative standard deviation (RSD) of les than 0.14% in 20 assays. The lifetime of the enzyme electrode was relative long and could be used continuously at 25 degrees C for 25 days. These results demonstrated that the enzyme electrode biosensor could be used to determine inosine and its derivatives specifically, rapidly, conveniently and economically.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biosensing Techniques
/
Sensitivity and Specificity
/
Inosine
/
Methods
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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