Modeling relationship between sequence characteristics of insecticidal crystal proteins and their inhibitory against Plutella xylostella / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1395-1400, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275372
ABSTRACT
The knowledge of the relationship between sequence characteristics of insecticidal crystal proteins (ICP) and their inhibitory against Plutella xylostella provided helpful information for the rational design of ICP with desirable activity against Plutella xylostella. The four key loops of ICP with determined activities against Plutella xylostella were selected to study the quantitative relationship between sequence characteristics and insecticidal activity. The first principle components' score vectors for 20 amino acids were assigned to converting amino acids into data. The six key sites X3, X9, X12, X13, X14 and X19 were predicted by stepwise regression method. The amino acids L/ X3, S/ X9, S/ X12, T/ X13, A/ X14 and G/ X19 found by partial least squares regression and second order polynomial models were predicted to increase the activity of ICP against Plutella xylostella.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bacterial Proteins
/
Molecular Sequence Data
/
Pest Control, Biological
/
Amino Acid Sequence
/
Sequence Analysis, Protein
/
Endotoxins
/
Genetics
/
Hemolysin Proteins
/
Insecticides
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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