Construction and expression analysis of micro-linear vector as a new general gene therapy vector / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1333-1339, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275381
ABSTRACT
The most difficult field in gene therapy is that vector system should offer both a means of successful transfection and a maximum of safety for the patient. Viral vectors and plasmid vectors are traditional vectors; they may cause unwanted immunological side effects resulting from the expression of nontherapeutic genes. Our aim is to develop a new general gene therapy vector which is suggested to be called as Micro-Linear Vector. The gene expression cassette is capped by our designed cap, including promoter, enhancer, objective gene, and RNA-stabilizing sequence, so it can defend the exnuclease in the eukaryotic cell, at the same time, DNA not encoding the objective gene is reduced to a minimum. The GFP gene is separated from the pEGFP-N3 plasmid, and acts as a reporter gene to construct the Micro-Linear Vector, then both the new vector and the plasmid are transfected to cells, the results are tested by fluorescence microscope and flow cytometry. The results show that the Micro-Linear Vector has a high effective of transfection and safety in 293, 3T3, CNE2 and B95-8 cell lines, at the same time it is less toxicity than the plasmid. We can get the rudiments of conclusion that Micro-Linear Vector has high effection of the transfection and more safety than tradition plasmid in eukaryotic cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Genetic Therapy
/
Promoter Regions, Genetic
/
3T3 Cells
/
CpG Islands
/
Cell Biology
/
Green Fluorescent Proteins
/
Genetic Vectors
/
Genetics
/
Kidney
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS