Cloning and expression of the pig skeletal muscle musclin gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1248-1252, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-275395
ABSTRACT
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Transcription Factors
/
Recombinant Fusion Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Open Reading Frames
/
Amino Acid Sequence
/
Cloning, Molecular
/
DNA, Complementary
/
Muscle, Skeletal
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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