Tea polyphenols reduces the apoptosis of spermatogenic cells in rats with experimental varicocele / 中华男科学杂志
National Journal of Andrology
;
(12): 702-707, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-276034
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of tea polyphenols (TP) on the apoptosis of germ cells in rats with experimental varicocele.</p><p><b>METHODS</b>Thirty-two adolescent male Wistar rats were randomly and equally divided into groups A (sham-operation), B (high-dose TP), C (low-dose TP), and D (experimental left varicocele). Experimental varicocele was induced by partial ligation of the left renal vein in the latter three groups of rats. The animals in groups A and D were fed with normal saline, while those in B and C with TP at 40 and 10 mg per kg per d, respectively, all for 4 weeks. Then, all the rats were sacrificed and the left testes harvested for determination of the expression of HIF-1, Bcl-2, Bax, CytC, and caspase-3 by immunohistochemistry and measurement of the apoptosis index (AI) of spermatogenic cells.</p><p><b>RESULTS</b>The expression of Bcl-2 was higher in groups B and C than in D but lower than in A (P < 0.05), and lower in C than in B (P < 0.05). However, the expressions of HIF-1, Bax, CytC, and caspase-3 were lower in groups B and C than in D but higher than in A (P < 0.05), and higher in C than in B (P < 0.05). The AI of spermatogenic cells was the lowest in group A, higher in D than in the other groups but lower in B than in C (P < 0.05).</p><p><b>CONCLUSION</b>TP can reduce the apoptosis of spermatogenic cells in a dose-dependent manner in varicocele rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Renal Veins
/
Spermatozoa
/
Tea
/
Testis
/
Varicocele
/
Random Allocation
/
Chemistry
/
Rats, Wistar
/
Apoptosis
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2015
Type:
Article
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