The analysis of promoters and the effective expression of exogenous proteins in a cold-adapted bacterium / 生物工程学报
Chinese Journal of Biotechnology
; (12): 415-422, 2008.
Article
in Zh
| WPRIM
| ID: wpr-276106
Responsible library:
WPRO
ABSTRACT
Based on the constructed promoter probe vectors that could replicate both in E. coli and in a cold-adapted bacterium, several candidate promoters were isolated and their activities were evaluated by RT-PCR. The transcription initiation sites and core sequence of promoters were determined by primer extension analysis. A low-temperature expression vector was constructed by using the strongest promoter and a thermolabile alpha-amylase gene was successfully overproduced under control of this promoter at low temperature (7 degrees C), while the secreted alpha-amylase amounted up to 35% of the total extracellular proteins. The expression system is expected to be useful for the production of thermolabile exogenous proteins at low temperatures.
Full text:
1
Index:
WPRIM
Main subject:
Bacterial Proteins
/
Transformation, Genetic
/
Acinetobacter
/
Molecular Sequence Data
/
Adaptation, Physiological
/
Base Sequence
/
Promoter Regions, Genetic
/
Cold Temperature
/
Escherichia coli
/
Alpha-Amylases
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article