Selection and identification of salt-tolerant variants of Taraxacum officinale / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 262-271, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-276129
ABSTRACT
In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Superoxide Dismutase
/
Genetic Variation
/
Sodium Chloride
/
Plant Leaves
/
Random Amplified Polymorphic DNA Technique
/
Taraxacum
/
Seedlings
/
Flavones
/
Culture Techniques
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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