The expression, purification and activity analysis of the rice chitinase gene in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 188-192, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-276142
ABSTRACT
The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oryza
/
Recombinant Proteins
/
Molecular Sequence Data
/
Chitin
/
Chitinases
/
Cloning, Molecular
/
Escherichia coli
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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