Application of spindle-view in the enucleation porcine of oocytes / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1140-1145, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-276147
ABSTRACT
The Spindle-view, a specialized instrument for observing spindle image, was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36, 42, 44, 48h, and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that (1) there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40-48 h under the instrument; (2) Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter' s method and two-step-squeezing method in the enucleated rates (95.5%, 42.1%, 74.2%, P < 0.0l) of absolutely removing nuclei matter; (3) the spindle images could be used to monitor the oocyte qualities.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Swine
/
Cell Nucleus
/
Cells, Cultured
/
Cytological Techniques
/
Cell Biology
/
Nuclear Transfer Techniques
/
Spindle Apparatus
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
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