Cloning and sequence analysis of SOCS-2 gene in pig / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1091-1096, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-276156
ABSTRACT
Total RNA was isolated from kidney of BaMei pig, a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5alpha, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp, is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25 kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Swine
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Cloning, Molecular
/
Sequence Homology
/
Sequence Analysis
/
Protein Structure, Secondary
/
DNA, Complementary
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2007
Type:
Article
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