Effect of heparin-derived oligosaccharide on vascular smooth muscle cell proliferation through inhibition of PKC-alpha expression / 药学学报
Acta Pharmaceutica Sinica
;
(12): 993-1000, 2012.
Article
in English
| WPRIM
| ID: wpr-276210
ABSTRACT
In this study, the effect of heparin-derived oligosaccharide (HDO) on bovine vascular smooth muscle cell (VSMC) proliferation and signal transduction mechanism involved were investigated. The levels of PKC-alpha protein and mRNA were determined by cell-based ELISA, RT-PCR, Western blotting and immunocytochemical methods. Meanwhile, mRNA levels of c-jun, c-myc and c-fos were assayed by RT-PCR method. The results showed that HDO inhibited newborn calf serum (NCS)-induced expression of PKC-alpha and proto-oncogenes, which may be one of the mechanisms for the inhibition of VSMC proliferation by HDO. Flow cytometry analysis indicated that HDO blocked NCS-induced cell cycle progression by arresting cells at G0/G1 phase. The results imply that HDO inhibits VSMC proliferation by moderating the gene level of PKC-alpha, eventually inhibiting proto-oncogene mRNA expression and blocking G1/S transition.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligosaccharides
/
Pharmacology
/
RNA, Messenger
/
Heparin
/
Signal Transduction
/
Cell Cycle
/
Cells, Cultured
/
G1 Phase
/
Proto-Oncogene Proteins c-myc
/
Proto-Oncogene Proteins c-jun
Limits:
Animals
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2012
Type:
Article
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