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Genomic analysis of a familial clustering of chronic hepatitis B patients / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 811-814, 2005.
Article in Chinese | WPRIM | ID: wpr-276344
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the peripheral blood monocyte (PBMC) gene expression profile in a familial clustering of patients with chronic hepatitis B (CHB).</p><p><b>METHODS</b>cRNA prepared from PBMC in a family with 5 CHB patients and 4 normal controls was hybridized to high-density oligouncleotide arrays (HG-U133A 2.0 Human GeneChips, Affymetrix), which interrogate the expression of approximately 22,000 human ESTs. Primary image obtained from scanning was analysed with a DNT software package. Real-time PCR was employed to confirm the gene chip results.</p><p><b>RESULTS</b>55 genes out of 22,000 ESTs were identified differently. Among the 55 genes 14 showed increased expression and 41 showed decreased expression in the familial clustering CHB patients compared with those in normal controls. Most of the genes (57%) were involved in immunity, inflammation, apoptosis, signaling transduction, and cell cycle.</p><p><b>CONCLUSION</b>These results suggest that the hosts with this broad range of gene expression alterations are susceptible to hepatic B infection.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pedigree / Leukocytes, Mononuclear / Cluster Analysis / Family Health / Genome / Hepatitis B, Chronic / Genetic Predisposition to Disease / Oligonucleotide Array Sequence Analysis / Gene Expression Profiling / Genetics Limits: Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hepatology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pedigree / Leukocytes, Mononuclear / Cluster Analysis / Family Health / Genome / Hepatitis B, Chronic / Genetic Predisposition to Disease / Oligonucleotide Array Sequence Analysis / Gene Expression Profiling / Genetics Limits: Female / Humans / Male Language: Chinese Journal: Chinese Journal of Hepatology Year: 2005 Type: Article