Gene expression analysis in liver tissue at a single cell level by nested polymerase chain reaction and laser microdissection / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 30-32, 2003.
Article
in Zh
| WPRIM
| ID: wpr-276511
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the measurements of gene expressing at a single hepatocyte level.</p><p><b>METHODS</b>Individual hepatocyte was isolated from cryostat tissue section using laser microdissection technique. To detect the mRNA expressed by single hepatocyte, RNA was extracted, reversely transcribed to cDNA and amplified by nested polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Single cell was microdissected from cryostat tissue using an ultraviolet laser micromanipulator. The RNA could be extracted from the isolated cell(s), and the RT-PCR production could be observed after electrophoresis, whose quantitation was compatible with the number of cells.</p><p><b>CONCLUSION</b>Combining laser microdissection and nested RT-PCR can monitor gene expression at a single cell level in vivo.</p>
Full text:
1
Index:
WPRIM
Main subject:
RNA, Messenger
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Hepatocytes
/
Dissection
/
Lasers
/
Metabolism
/
Methods
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article