Influence of mutation in HBV precore region on the expression of HLA-I in HepG2 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 351-353, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-276570
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of host cellular HLA-I molecules expression by HBV precore region mutants.</p><p><b>METHODS</b>The plasmids of the wild type of HBV precore region and the mutants of A83 and A83/A86 were constructed, and then transected into HepG2 cells. The biological activity of HBV precore gene in the host cells was identified. The expression of HLA-I molecules was detected by flow cytometric analysis.</p><p><b>RESULTS</b>DNA segments similar with HBV precore gene size and HBeAg were detected by PCR and ELISA, respectively. The fluorescence intensity of HLA-I on host cells was different wild type being 1.3; A83, 17.6; and A83/A86, 7.3.</p><p><b>CONCLUSIONS</b>HBV precore gene hot-spot mutants in vitro can increase the expression of HLA-I molecules on host cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Viral
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Allergy and Immunology
/
Hep G2 Cells
/
Genetics
/
Hepatitis B e Antigens
/
HLA Antigens
/
Mutation
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS