HPLC analysis of alfatoxins in medicinal herb extracts by immunoaffinity column cleanup and post-column bromination / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 182-184, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-276620
ABSTRACT
<p><b>OBJECTIVE</b>To develop a new and accurate method to quantify aflatoxins in medicinal herbs.</p><p><b>METHOD</b>This method consists of sample extraction by using MeOH-H2O (73), followed by clean-up with an immunoaffinity column, and finally HPLC determination with fluorescence detection. Aflatoxins B1 and G1 are determined as their bromine derivatives, produced in an on-line post-column derivatization system.</p><p><b>RESULT</b>The overall average recoveries for different medicinal herbs spiked at two levels of standards were from 90.4% to 99.7%. The detection limit was 0.06 microg x kg(-1) for aflatoxins G2 and B2, and 0.20 microg x kg(-1) for aflatoxins G1 and B1.</p><p><b>CONCLUSION</b>The use of immunoaffinity column provides excellent cleanup of interfering substances. The method has been applied successfully to analyze 96 natural drugs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, Affinity
/
Chromatography, High Pressure Liquid
/
Aflatoxin B1
/
Aflatoxins
/
Codonopsis
/
Prunus
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article
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