Pharmacokinetics of oxymatrine and its metabolite in beagle dogs by LC-MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 133-136, 2005.
Article
in Zh
| WPRIM
| ID: wpr-276629
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish LC-MS method in the determination of oxymatrine and its metabolite in plasma and investigate their pharmacokinetics in beagle dogs.</p><p><b>METHOD</b>Lichrospher C18 column (4.6 mm x 250 mm, 5 microm) was used as the analytical column maintained at 25 degrees C. The mobile phase consisted of 10 mmol x L(-1) CH3COONH4 and CH3OH (25:75). Flow rate was 1 mL x min(-1). Electrospray ionization (ESI) was carried out. The ESI ion source was set in positive ion polasity mode. The selective ion monitoring (SIM) was set at m/z 265.1 and 249.2.</p><p><b>RESULT</b>The linearity ranged from 2 to 5000 ng x mL(-1) (r = 0.9991). The detection of oxymatrine and its metabolite were 0.6 and 0.3 ng x mL(-1). The RSD(%) within day and between day was less than 4.7%. The recovery of this method was more than 96.5%. The disposition was conformed to a two-compartment model. The T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of oxymatrine were (5.5+/-1.58) h, (1.0+/-0.30) h, (2418.3 +/-970.78) ng x mL(-1), (3.2+/-0.64) h, (5797.4+/-908.16) ng x mL(-1) x h accordingly. The corresponding T(1/2), Tmax, Cmax, MRT, AUC(0-->24 h) of matrine were (9.8+/-2.77) h, (1.9+/-1.09) h, (1532.4+/-494.86) ng x mL(-1), (4.4+/-1.97) h, (5530.5+/-1042.65) ng x mL(-1) x h.</p><p><b>CONCLUSION</b>This assay was highly sensitive, rapid, simple and specific enough for determining concentrations of oxymatrine and its metabolite matrine in plasma of beagle dog.</p>
Full text:
1
Index:
WPRIM
Main subject:
Plants, Medicinal
/
Quinolizines
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Blood
/
Pharmacokinetics
/
Chemistry
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Administration, Oral
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Chromatography, Liquid
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Area Under Curve
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Spectrometry, Mass, Electrospray Ionization
/
Sophora
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article