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Determination of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Radix Notoginseng and its preparation by HPLC-ELSD / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-276635
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the quantitative method of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Radix Notoginseng and its preparation Xuesaitong injection by HPLC-ELSD.</p><p><b>METHOD</b>The column was packed with 5 microm Diamonsil C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L x min(-1).</p><p><b>RESULT</b>The linear ranges of the three components were 0.456-2.25 microg, 1.47-7.38 microg and 1.20-6.03 microg respectively. The average recoveries of the three components in Radix Notoginseng were 97.1% (RSD 1.9%), 96.8% (RSD 2.0%), 97.0% (RSD 2.2%) respectively; in Xuesaitong Injection were 98.7% (RSD 1.9%), 98.5% (RSD 1.8%), 98.1% (RSD 1.4%) respectively.</p><p><b>CONCLUSION</b>It was proved that the method was reliable, simple, and precise, that could be used for quality control.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Plant Roots / Ginsenosides / Panax / Injections / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Quality Control / Drugs, Chinese Herbal / Chemistry / Chromatography, High Pressure Liquid / Plant Roots / Ginsenosides / Panax / Injections / Methods Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2005 Type: Article