Injury in renal proximal tubular epithelial cells induced by aristololactam I / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 78-83, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-276660
ABSTRACT
<p><b>OBJECTIVE</b>To study whether aristololactam I (AL-I) induces injury in human renal proximal tubular epithelial cells.</p><p><b>METHOD</b>Cultured human renal proximal tubular epithelial cell line HK-2 was used as the subject. Aristolochic Acid I (AA-I) was used as a positive control. Cell toxicity of AL-I was detected by LDH releasing rate. Cell apoptosis was evaluated by cellular morphology, DNA content and expression of cell membrane phosphatidylserine (PS). The secretion level of fibronectin (FN) and TGF-beta1 in HK-2 cells were assayed by ELISA.</p><p><b>RESULT</b>AL-I had a direct toxicity on HK-2 in a dose dependent manner from 2.5 mg x mL(-1) to 20 mg x mL(-1); In these range of concentration, AL-I could induce cell apoptosis which was detectable by measurements of morphology, DNA content and expression of PS. AL-I could stimulate the secretion of FN and TGF-beta1. The potency of AL-I cell toxicity was higher than AA-I at the same concentration. The effects of AL-I on apoptosis, secretion of FN and TGF-beta1 were all weaker than AA-I.</p><p><b>CONCLUSION</b>AL-I as one metabolite of AA-I in vivo induces direct injury in renal proximal tubular cells. Its effects are similar to those of AA-I. AL-I may be one of toxic metabolites in Chinese herbs containing AA which participate in renal damage and fibrosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
DNA
/
Cell Line
/
Cell Membrane
/
Chemistry
/
Apoptosis
/
Aristolochia
/
Cell Biology
/
Aristolochic Acids
/
Diploidy
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2004
Type:
Article
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