Adenovirus-mediated PDCD5 gene transfer sensitizes apoptosis of K562 cells induced by etoposide / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 936-940, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-276788
ABSTRACT
This study was purposed to investigate the effect of adenovirus-mediated transfer of PDCD5 gene on apoptosis of K562 cells induced by etoposide. Recombinant adenovirus PDCD5 (Ad-PDCD5), control vectors Ad-null and Ad-eGFP were constructed by AdMax vector system respectively. After K562 cells were transfected by Ad-PDCD5, Ad-null or Ad-eGFP with different multiplicity of infection (MOI), the expression level of the PDCD5 gene was examined by RQ-RT-PCR assay. The effects of etoposide in combination with Ad-PDCD5 on the proliferation and apoptosis of K562 cells were measured by using MTT assay and flow cytometry with Annexin-V-FITC/PI dual labeling technique, respectively. The results showed that the transfection efficiencies of Ad-eGFP in K562, Jurkat and CEM cells ranged from 60% to 86%. Expression level of PDCD5 gene in K562 cells was evidently increased following transfection with Ad-PDCD5. The Ad-PDCD5 synergistically enhanced the apoptotic percentage of K562 cells induced by VP-16, as compared with that of Ad-null + VP16 and VP-16 alone respectively. It is concluded that Ad-PDCD5 may be a potential agent for enhancing the chemotherapy effect.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Recombinant Proteins
/
RNA, Messenger
/
Transfection
/
Adenoviridae
/
Apoptosis
/
K562 Cells
/
Apoptosis Regulatory Proteins
/
Etoposide
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS